Fluorescence Based Protein Detection Testing Pre-Conference Lab at the 2015 IAHCSMM

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IAHCSMM 50th Annual Conference & Expo
Saturday, May 2, 2015 (Pre-Conference Labs and Workshops)

The following Hands-On Lab will repeat two times at 9am and 10:15am

FLUORESCENCE BASED PROTEIN DETECTION TESTING
Dr. Perrett, Thomas Overbey, Rose Seavey – Ultra Clean Systems
(Lab)

The detection of residual proteins is considered to be a necessary step for the validation of decontamination procedures in hospital SPDs.

Protein determinations are conducted off-instrument by either wet swabbing part of the instrument or by soaking the instrument in a detergent solution. Previous studies have demonstrated that significant amounts of adsorbed BSA or fibrinogen, (a relatively hydrophobic protein with some similar characteristics to prion proteins) can remain on the instrument after swabbing with water or detergent solutions.

A suggested alternative approach is the in-situ detection of residual proteins on the actual instruments. Proteins adsorbed onto surfaces can be directly quantified by a number of possible techniques. Many such techniques are at present far too complex and expensive for routine use.

Another technique is through the use of fluorescence imaging since it can offer considerably more sensitivity.

This lab will provide a thorough step-by-step demonstration of fluorescence based protein test detection and follow-up discussion by the inventors of the only system of its kind.

Come see us there. Seating is very limited!


 

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